Last data update: 24 January 2024 16:39 CET
Host name: Escherichia coli K12 JM109(DE3) (LMBP 2406)
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| Species: | Escherichia coli |
| Group: | K12 |
| Strain designation: | JM109(DE3) |
| Accession number: | LMBP 2406 |
| Depositor: | This host strain was deposited by Prof. Dr R. Beyaert1 2. It was obtained from Dr J. Messing 3. 1 VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium 2 Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium 3 Waksman Institute, Rutgers, State University of New Jersey, Piscataway, USA |
| Containment level: | This strain has been assigned the containment level Class 1 following the European Directive 2009/41/EC on the contained use of genetically modified organisms, and its updates (see also the Belgian risk group classification). |
| Medium: | LB-Lennox |
| Selection marker: | Nalidixic acid |
| Temperature: | 37°C |
| Genotype: | [F´ traD36 proAB laqIqZΔM15] endA1 recA1 gyrA96 thi hsdR17 relA1 supE44 Δ(lac-proAB) λ(DE3 [lacI lacUV5-T7 gene 1 ind1 sam7 nin5]) |
| Phenotype: | NalR rk- mk+ |
| Properties: | The strain contains DE3, a λ prophage carrying the T7 RNA polymerase gene under control of the lac UV5 promoter and lacIq. IPTG is required to induce expression of the T7 RNA polymerase. Protein expression from transforming plasmids containing a T7 promoter-driven expression system is repressed until IPTG induction of T7 RNA polymerase expression occurs. |
| Further information: | There is a genome sequence of the parental JM109 strain available at Genbank (Accession number: NZ_RYWX00000000.1). The nalidixic acid resistance has been experimentally verified by BCCM/GeneCorner. |
| Original reference: | - |
| Related reference: | Yanisch-Perron et al., Gene 33 (1985), 103-119 [PMID: 2985470] |
| Restricted use: | - BCCM MTA |
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Refer in your Materials and Methods: |
Escherichia coli K12 JM109(DE3) (LMBP 2406) is available at BCCM/GeneCorner. It was deposited by Prof. Dr R. Beyaert. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.